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Dr. Shashwat Sharad

M.Sc., Ph.D.

Principal Research Officer

Rajiv Gandhi Cancer Institute, Sector - 5, Rohini, Delhi - 110 085

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About

Dr. Shashwat Sharad is an established cancer and biomedical researcher with a strong academic/administrative track record in the cancer biology and translational research. Dr. Sharad integrates research activities within Basic and Translational Research, focusing on diagnosing, early detection, and treating human diseases. His research contributions in the field of cancer biology and genetics with special focus on the fundamental mechanisms of oncogene and tumor suppressor gene alterations and prostate cancer basic and translational research have been recognized internationally.

Dr. Sharad has established the unique bio-resources under Dr. Shiv Srivastava’s leadership at Center for Prostate Disease Research (CPDR), Murtha Cancer Center Research Program, and Uniformed Services University of Health Sciences-Walter Reed Surgery, Maryland, USA. Dr. Sharad's current research focuses on: (1) defining prostate cancer-specific gene/protein alterations and hormonal mechanisms using state-of-the-art gene expression profiling, positional cloning and functional genomics approaches (2) developing and evaluating novel technologies for improving diagnosis and prognosis of prostate cancer using blood and urine based prostate cancer-specific gene expression or proteomics profiles.

As the CPDR Staff Scientific and Assistant Professor of Surgery, Dr. Sharad has played a central role in the successful outcome of the multi-disciplinary translational research effort between urologists, cancer biologists, pathologists, epidemiologists and bio-and medical informaticians.

Dr. Sharad has been the recipient of several research fellowships and grant awards. Dr. Sharad has been actively involved in teaching and educating high school and college students, graduate students, medical students, postdoctoral fellows, and urology residents. Dr. Sharad actively volunteers his scientific expertise to the international cancer research community by serving as a reviewer and editorial board member for leading journals in the field. Dr. Sharad is an entrepreneur and business strategist whose expertise lies in healthcare solutions. He developed an Innovative Optical-Sensor-Based, Low-Cost, Rapid Diagnostic Test to Reduce Antimicrobial Resistance- A point-of-care device for testing antibiotic sensitivity of pathogens found in the human urinary tract in less than 4 hours.

PROFESSIONAL JOURNEY

ACHIEVEMENTS
  • Developed an Innovative Optical-Sensor-Based, Low-Cost, Rapid Diagnostic Test to Reduce Antimicrobial Resistance- A point-of-care device for testing antibiotic sensitivity of pathogens found in human urinary tract.
  • Developed Herbal formulation to prevent transmission of SARS-COV-2 virus and their variants. Involved in molecular, biochemical, and genetic evidence for elucidating mechanism of action of formulations derived from Indian Medicinal plants.
  • Developed a novel conditional double knockout mouse model to study the biological function of prostate cancer tumorigenesis.
AWARDS Grants:
  • Identification of distinct biomarker candidate genes (PMEPA1 isoforms) to predict prostate cancer metastasis and castration resistance in a racially diverse, military cohort of prostate cancer patients in context of hormone (androgen/TGF-β) regulation (Principal Investigator)
  • ER stress induced radiation resistance and pro-survival pathways as potential biomarkers and therapeutic targets of prostate cancer (Co- Principal Investigator)
  • The Complex Biological and Social Determinant that Affect Development of Cardiovascular and Pulmonary Comorbid Conditions and Associated Progression of Prostate Cancer on Androgen deprivation therapy in a longitudinal military cohort. (Co-Principal Investigator)
  • Interplay of social determinants and the epigenome and metabolome on prostate cancer progression in a longitudinal military cohort. (Principal Investigator)
Research Grants Awarded
  • 2020-2021 Defining the novel epigenomic and transcriptomic landscape for early detection, risk assessment and progression in the context of androgen receptor splicing, TGF-β signaling of castration-resistant and/or metastatic prostate cancer in ethnically diverse longitudinal military cohort. (Principal Investigator) From CPDR-USU Core
  • 2009-2013 DRDO/NPMASS- Development of an indigenous chip for testing the sensitivity of pathogens in human urine for antibiotics. (Co-Investigator)
  • 2008-2011 ICMR- Identification of Genetic Loci Associated with Risk for T2DM in Indians (Co-PI)
  • 2009-2010 DST-Understanding of role of mu opiate receptor in Immuno-regulatory functions of Glial cells using Transcriptome profiling. (Principal Investigator)
  • 2006-2007 RSSDI-Plasma Levels of the cytokines MCP-1 & IL-8 in subjects with metabolic syndrome and obesity. (Principal Investigator)
Fellowships
  • 2009-2015 Post-doctoral Fellowship from The Henry M. Jackson Foundation for the Advancement of Military Medicine, Maryland, USA
  • 2007-2009 Research Associate Fellowship from Indian Council of Medical Research, New Delhi, India
  • 2004-2007Senior Research Fellowship from Indian Council of Medical Research, New Delhi
  • 1993-1995 State Level Merit Scholarship for Higher Secondary School (grade 11th-12th) by State Government Uttar Pradesh, India
  • 1991-1993 State Level Merit Scholarship for High School (grade 9th-10th) by State Government Uttar Pradesh, India
  • 1988-1991 District level Merit Fellowship for Middle School (grade 6th to 8th) by State Government Uttar Pradesh, India
  • April 26 -28, 2011 Selected for RNAi Screening Workshop being held in Baltimore, Maryland, USA at Johns Hopkins University School of Medicine sponsored by Sigma.
  • July 13 -24, 2009 Prestigious REKEN Fellowship to attend the Summer Lecture Course, RIKEN Brain Science Institute, Saitama, Japan.
  • Nov 14-19, 2008 Travel award to present a poster in Early Career Poster Session at the NIDA “Frontiers in Addiction Research” satellite event associated with the Society for Neuroscience annual meeting in Washington DC, USA.
  • Oct 15-17, 2008 Award to present a paper in Human Genome Variation Meeting held at Toronto, Canada.
  • Sept 8-11, 2008 Travel grant to present an oral podium presentation in the 6th International Symposium on Experimental and Clinical Neurobiology, Kosice, Slovakia.
  • July 10-12, 2007 Travel, Registration and Accommodation support from Society for Neuroscience, Washington DC, USA to attend The Neurodegenerative Diseases Workshop held at Melbourne
  • July 12-17, 2007 7th IBRO World Congress of Neuroscience to present a paper entitled "Correlation of circulatory immunoglobulin levels with mu opiate receptor allele type".
  • Nov 30-Dec 2, 2006 IBRO/FAONS travel grant to attend and present the paper in The 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, held at University of Hong Kong, Hong Kong.
  • Sept 2-9, 2006 IBRO (International Brain research Organization) Fellowship to attend the 8th IBRO School of Neuroscience at Tata Institute of Fundamental Research, Mumbai, India.
PUBLICATIONS Prostate Cancer
  1. Sharad S*, Hua Li*, Talai Barbiev, Wei Huang, Yingjie Song, Denise Young, Issabell Sesterhenn, Inger Rosner, Albert Dobi, Shiv Srivastava, Taduru Sreenath. Deletion of Pmepa1 gene confers increased cell proliferation in mouse prostate epithelium and leads to enhanced Ar signaling. Under submission.
  2. Sharad S*, Sztupinszki Z, Szallasi Z, Shriver CD, Srinivasan A, Hua Li*. PMEPA1 Gene Isoforms Predict Aggressive Disease Progression in Non-prostate Solid Tumors of Breast, Lung and Colon. Submitted Scientific Report 2022
  3. Sharad S, Allemang TC, Li H, Nousome D, Ku AT, Whitlock NC, Sowalsky AG, Cullen J, Sesterhenn IA, McLeod DG, Srivastava S, Dobi A. l. Age and Tumor Differentiation-Associated Gene Expression Based Analysis of Non-Familial Prostate Cancers. Front Oncol. 2021
  4. Sharad S, Dillman AA, Sztupinszki ZM, Szallasi Z, Rosner I, Cullen J, Srivastava S, Srinivasan A, Li H. Characterization of unique PMEPA1 gene splice variants (isoforms d and e) from RNA Seq profiling provides novel insights into prognostic evaluation of prostate cancer. 2020
  5. Sharad S, Sztupinszki ZM, Chen Y, Kuo C, Ravindranath L, Szallasi Z, Petrovics G, Sreenath TL, Dobi A, Rosner IL, Srinivasan A, Srivastava S, Cullen J, Li H. Analysis of PMEPA1 Isoforms (a and b) as Selective Inhibitors of Androgen and TGF-β Signaling Reveals Distinct Biological and Prognostic Features in Prostate Cancer. Cancers (Basel). 2019
  6. Katta S, Srivastava A, Thangapazham RL, Rosner IL, Cullen J, Li H, Sharad S. Curcumin-Gene Expression Response in Hormone Dependent and Independent Metastatic Prostate Cancer Cells. Int J Mol Sci. 2019
  7. Sreenath TL, Macalindong SS, Mikhalkevich N, Sharad S, Mohamed A, Young D, Borbiev T, Xavier C, Gupta R, Jamal M, Babcock K, Tan SH, Nevalainen MT, Dobi A, Petrovics G, Sesterhenn IA, Rosner IL, Bieberich CJ, Nelson P, Vasioukhin V, Srivastava S. ETS Related Gene mediated Androgen Receptor Aggregation and Endoplasmic Reticulum Stress in Prostate Cancer Development. Sci Rep. 2017
  8. Sharad S. Surgical Management Discrepancies of Prostate Cancer. Journal Prostate Cancer. 2016
  9. Kumar P, Sharad S, Petrovics G, Mohamed A, Dobi A, Sreenath TL, Srivastava S, Biswas R. Loss of miR-449a in ERG-associated prostate cancer promotes the invasive phenotype by inducing SIRT1. Oncotarget, 2016
  10. Li H, Mohamed AA, Sharad S, Umeda E, Song Y, Young D, Petrovics G, McLeod DG, Sesterhenn IA, Sreenath T, Dobi A, Srivastava S. Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN. Oncotarget, 2015
  11. Sharad S, Ravindranath L, Haffner MC, Li H, Yan W, Sesterhenn IA, Chen Y, Ali A, Srinivasan A, McLeod DG, Yegnasubramanian S, Srivastava S, Dobi A, Petrovics G. Methylation of the PMEPA1 gene, a negative regulator of the androgen receptor in prostate cancer. Epigenetics, 2014
  12. Rastogi A, Tan SH, Banerjee S, Sharad S, Kagan J, Srivastava S, McLeod DG, Srivastava S, Srinivasan A. ERG monoclonal antibody in the diagnosis and biological stratification of prostate cancer: delineation of minimal epitope, critical residues for binding, and molecular basis of specificity. Monoclon Antib Immunodiagn Immunother, 2014
  13. Sharad S, Srivastava A, Ravulapalli S, Parker P, Chen Y, Li H, Petrovics G, Dobi A: Prostate cancer gene expression signature of patients with high body mass index. Prostate Cancer and Prostatic Dis. 2011
Book Chapter
  1. Book “Antisense Therapy” by Shashwat Sharad (Eds.) 2019, InTech Publisher. ISBN: 978-1-78984-532-7. (https://www.intechopen.com/books/antisense-therapy)
  2. Shashwat Sharad, Taduru Sreenath, Shiv Srivastava and Albert Dobi. Cancer Genome Methylation: Biology, Biomarker and Therapeutic Opportunities, In “Biomarkers in Cancer Screening and Early Detection”, by Sudhir Srivastava (Eds.) 2017, Wiley-Blackwell. ISBN: 978-1-118-46880-7.
  3. Suman Kapur, Anuradha Pal and Shashwat Sharad. Role of Opioidergic System in Humoral Immune Response, In “Immunosuppression: Role in Health and Disease”, by. Suman Kapur (Eds.), 2012 InTech Publisher. ISBN: 978-953-51-0152-9.
  4. Vaddula BR, Kumar D, Sharad S, Dube U, Kapur S. Multi-component reactions in drug discovery In “Advances in Experimental Medicine and Biology”, by Maxim A. Mironov (Eds.) 2011 ISBN: 978-1-4419-7269-9 Publisher Springer, New York Dordrecht Heidelberg London. 2011; 699:75-106.
Published Genes submitted to NCBI: NCBI Gene References Homo sapiens crystallin, gamma B (CRYGB), mRNA, NM_005210.3 GI:148491073; crystallin, gamma A (CRYGA), mRNA NM_014617.3 GI:148491056; clone BITS-SSSK-05 mutant opioid receptor mu (OPRM1) gene, exon 1 and partial cds, EU826063.1 GI:215276401; clone BITS-SSSK-04 mutant opioid receptor mu (OPRM1) gene, exon 1 and partial cds, EU826062.1 GI:215276399; clone BITS-SSSK-03 mutant opioid receptor mu (OPRM1) gene, exon 1 and partial cds, EU826061.1 GI:215276397; clone BITS-SSSK-02 mutant opioid receptor mu (OPRM1) gene, exon 1 and partial cds, EU826060.1 GI:215276395; clone BITS-SSSK-01 opioid receptor mu (OPRM1) gene, exon 1 and partial cds, EU826059.1 GI:215276393 Infectious Disease
  1. Gehani M, Kapur S, Madhuri SD, Pittala VP, Korvi SK, Kammili N, Sharad S. Effectiveness of antenatal screening of asymptomatic bacteriuria in reduction of prematurity and low birth weight: Evaluating a point-of-care rapid test in a pragmatic randomized controlled study, 2021
  2. Kapur S, Gupta S, Sharad S, Shastry S, Padmavathi DV, Growing antibiotic resistance in uropathogens due to irrational use of antibiotics. Journal of Antimicrobials. Photon, 2013
  3. Kapur S, Gehani M, Kammili N, Bhardwaj P, Nag V, Devara SM, Sharad S. Clinical Validation of Innovative Optical-Sensor-Based, Low-Cost, Rapid Diagnostic Test to Reduce Antimicrobial Resistance. J Clin Med. 2019
  4. Kumar D, Reddy VB, Sharad S, Dube U, Kapur S. A facile one-pot green synthesis and antibacterial activity of 2-amino-4H-pyrans and 2-amino-5-oxo-5,6,7,8-tetrahydro-4H-chromenes. Eur J Med Chem, 2009
Neuropsychiatry
  1. Kapoor M, Kapur S, Mehra S, Dube U, Sharad S, Sidhu S. Genetic variation of D7S1875 dinucleotide repeats in the leptin gene increases risk for depression: A population-based case-control study in India. Depress and Anxiety, 2009
  2. Sharad S, Gupta AK, Singh RA, Kapoor M, Kapur S. Correlation of Circulatory Immunoglobulin Levels with Mu Opiate Receptor- Allele Type. Indian J Biochem & Biophys, 2007
  3. Kapur S, Sharad S, Singh RA, Gupta AK. A118G polymorphism in mu opioid receptor gene (oprm1): association with opiate addiction in subjects of Indian origin. J Integr Neurosci, 2007
  4. Kapur S, Sharad S, Kapoor M, Bala K: APOE Genotypes: Risk Factor for Alzheimer's disease. Ind. Acad. Clin. 2006
Metabolic Disorders
  1. Mujawdiya PK, Sharma P, Sharad S, Kapur S. Reversal of Increase in Intestinal Permeability by Mangifera indica Seed Kernel Extract in High-Fat Diet-Induced Obese Mice. Pharmaceuticals (Basel). 2020
  2. Kapur S, Pal A, Gurjar S, Pavan VVS, Sharad S. Isolation and characterization of secondary metabolites “Saponins” from explants of Indian medicinal plant Chlorophytum borivilianum, IJPFR, 2011
  3. Kapur S, Mehra S, Gajjar D, Vasavada A, Kapoor M, Sharad S, Alapure B, Rajkumar S. Analysis of Single nucleotide polymorphisms of γ – Crystallin A and B genes in control population of Western Indian origin. Indian J Ophthalmol, 2009
  4. Jhanjaria A, Kapur S, Agrawal RP, Kapoor M, Sharad S, Dube U, Mohta N, Kochar DK. First study of aryl hydrocarbon nuclear translocator gene in Indians: Correlation with resistance to diabetes in Raika community. Diabetes Res Clin Pract, 2008

Specialized Areas

  • Wide expertise in laboratory bench studies, patient studies, writing scientific manuscripts, grant proposals and maintaining research lab operations.
  • Experience in cell and molecular biology, epigenetics, reverse genetics, plasmid construction, sequencing, gene cloning, protein expression analysis, PCR, QRT-PCR, transfection, confocal and florescence imaging, cell culture, plaque assay, animal studies, immunoassays; ELISA, immunofluorescence, immunohistochemistry, western blot, dot blot.

Educational Background

  • Ph.D., Biochemistry Sam Higginbottom University of Agriculture, Technology & Sciences, Uttar Pradesh, India in collaboration with Birla Institute of Technology and Science, Pilani, Rajasthan, India - 2007
  • M.Sc., Microbiology Dr. Ram Manohar Lohia Avadh University, Ayodhya, Uttar Pradesh, India - 2001
  • B.Sc. Zoology, Botany, Chemistry, Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India - 1999
  • B.A. Honors Public Administration, Indira Gandhi National Open University, Delhi, India - 2000

Experience

  • 2019 to 2022 Assistant Professor of Surgery, F. Edward Hébert School of Medicine, Department of Surgery, Uniformed Services University, Bethesda, MD
  • 2012-Till Date Member Research Advisory Board, Vivekananda Global University, Jaipur, India 2020 to 2022 Staff Scientist II, Center for Prostate Disease Research, Uniformed Services University-Walter Reed Surgery, Bethesda, MD
  • 2015 to 2019 Staff Scientist I, Center for Prostate Disease Research, Uniformed Services University-Walter Reed Surgery, Bethesda, MD
  • 2009-2015 Postdoctoral Fellow, Center for Prostate Disease Research, Uniformed Services University-Walter Reed Surgery, Bethesda, MD
  • 2007-2009 Research Associate, Birla Institute of Technology and Science, Pilani, India 2009-2009 Senior Lecturer: Biotechnology, Meerut Institute of Engineering and Technology, India (Postgraduate and Undergraduate Teaching and Research) Courses: Post Graduate and Undergraduate Biotechnology courses, Cell and Molecular Biology, Microbial and Industrial Biotechnology, Biochemistry, Molecular Dynamics & Bioenergetics, Animal Cell and Tissue Culture
  • 2004-2007 Course Instructor, Biological Sciences Group, Birla Institute of Technology and Science, Pilani, India (Postgraduate and Undergraduate Teaching and Research) Courses: Microbiology, Measurement Techniques I, Genetic Engineering, Instrumental Method of Analysis, Laboratory, Cell and Tissue Culture, Biochemistry, Stem Cell and Regenerative Biology, Cell Biology 2004-2007 Senior Research Fellow, (Indian Council of Medical Research, New Delhi, India) at Biological Sciences Birla Institute of Technology and Science, Pilani, India
  • 2002-2004 Research Assistant, (Indian Council of Medical Research Fellowship, New Delhi, India) at Dept. of Neurobiochemistry, Institute of Human Behavior and Allied Sciences, India
  • 2001-2002 Project Assistant, Department of Human Genetics, National Institute of Mental Health and Neurosciences, Bangalore, India
 

Membership

  • Sigma Xi
  • Murtha Cancer Center
  • Society for Neuroscience (SFN), Washington DC, USA,
  • American Association for Cancer Research (AACR), Philadelphia, PA, USA
  • American Society of Clinical Oncology (ASCO), Alexandria, VA, USA
  • The Epigenetics Society (ES)Epigenetics Society
  • American Urological Association (AUA), Linthicum Heights, MD, USA

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